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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Equine leucocytes.
- 亚型:
IgG1
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Horse
- 保质期:
自发货之日起 12 个月
- 目录编号:
CVS5
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, F, IP
- 浓度:
IgG concentration 1.0 mg/ml
- 抗体名:
CD5 antibody | CVS5
- 规格:
0.1 mg
Mouse anti Horse CD5 antibody, clone CVS5 recognizes the equine CD5 antigen, a single-pass type I membrane protein with an approximate molecular weight of 69 kDa. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.
In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/25 | 1/200 |
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD5 antibody
- Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
Immunology. 74 (2): 251-7. - Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. - Moyo, N.A. et al. (2013) Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression.
Immunology. 139 (4): 472-83. - Mayall, S. et al. (2001) The anti-human CD21 antibody, BU33, identifies equine B cells.
J Comp Pathol. 124 :83-7. - Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
Vet Immunol Immunopathol. 60: 15-31. - Colbath, A.C. et al. (2017) Autologous and Allogeneic Equine Mesenchymal Stem Cells Exhibit Equivalent Immunomodulatory Properties In Vitro.
Stem Cells Dev. 26 (7): 503-11. - Ziegler, A. et al. (2016) Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.
BMC Vet Res. 12 (1): 254. - Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7.
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Mouse anti Horse CD5,clone CVS5
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