- 询价
- BIO-RAD
- MCA2125F
- 2025年10月17日
- F
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
RIL-2 propagated NK1.1+ cells from C57BL/6 mice.
- 亚型：
IgM
- 形态：
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Mouse
- 保质期：
自发货之日起 12 个月
- 目录编号：
DX5
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgM concentration 0.1 mg/ml
- 抗体名：
CD49b antibody | DX5
- 规格：
0.1 mg
Rat anti Mouse CD49b monoclonal antibody, clone DX5 recognizes mouse CD49b, also known as Very Late Antigen-2 (VLA-2) or alpha-2 integrin. CD49b is a ~160kDa single pass type I membrane protein expressed by NK cells and a small subset of T-cells (Pellicci et al. 2005).
Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).
Rat anti mouse CD49b, clone DX5 stains NK cells from all mouse strains tested including Balb/c mice (Arese et al. 2001). CD49b acts as a receptor for collagen, laminin, fibronectin and E-cadherin (Carafoli et al. 2013).
储存信息
This product is shipped at ambient temperature.
Store at +4°C only.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4°C only.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
-
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
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文献和实验该产品被引用文献
References for CD49b antibody
- Arase, H. et al. (2001) Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).
J Immunol. 167 (3): 1141-4. - Jacobs, N. et al. (2006) Intradermal immune response after infection with Vaccinia virus.
J Gen Virol. 87: 1157-61. - Yin, Y. et al. (2013) Phosphatidylserine-targeting antibody induces M1 macrophage polarization and promotes myeloid-derived suppressor cell differentiation.
Cancer Immunol Res. 1 (4): 256-68. - Lin, C.Y. et al. (2008) Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.
Cancer Lett. 272: 285-95. - Thomson, R.C. et al. (2008) Expansion and activation of NK cell populations in a gammaherpesvirus infection.
Scand J Immunol. 67: 489-95. - Pais, R. et al. (2012) An intracameral injection of antigen induces in situ chemokines and cytokines required for the generation of circulating immunoregulatory monocytes.
PLoS One. 7: e43182.
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文献支持
Rat anti Mouse CD49b:FITC，clone DX5
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