- 询价
- BIO-RAD
- MCA2308F
- 2025年10月17日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine alveolar macrophages.
- 亚型：
IgG2b
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
BL1H8
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD11a antibody | BL1H8
- 规格：
0.1 mg
Mouse anti Pig CD11a, clone BL1H8 recognizes porcine CD11a, binding to a conformational epitope on the alpha chain of LFA-1 (CD11a/CD18), expressed on a range of cells including peripheral blood lymphocytes, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 immunoprecipitates two bands of 170 kDa (CD11a) and 95 kDa (CD18) from porcine alveolar lysates, pre-clearance of the lysates completely removed the 170 kDa band , strongly suggesting the epitope for this antibody is located on the CD11a chain, although some involvement of residues on the LFA-1 β chain could not be formally ruled out (Alvarez et al. 2000). Mouse anti Pig CD11a, clone BL1H8 was clustered as porcine CD11a at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001).
Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000).
Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD11a antibody
- Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7. - Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
Xenotransplantation. 7 (4): 258-66. - Van de Walle, G.R. et al. (2003) Transmission of pseudo* from immune-masked blood monocytes to endothelial cells.
J Gen Virol. 84 (Pt 3): 629-37. - Vanden Bergh, P.G. et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.
Vet Res. 40: 1-10. - Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244. - Wang, Y. et al. (2019) Luteolin Partially Inhibits LFA-1 Expression in Neutrophils Through the ERK Pathway.
Inflammation. 42 (1): 365-74.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54. - Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
Mol Immunol. 66 (1): 3-13.
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文献支持
Mouse anti Pig CD11a:FITC，clone BL1H8
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