- 询价
- BIO-RAD
- MCA4754F
- 2025年10月17日
- F
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
RBL-2H3 rat basophilic leukaemia cell line.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
AD1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1mg/ml
- 抗体名：
CD63 antibody | AD1
- 规格：
0.1 mg/25 µg
| 规格： | 0.1 mg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 25 µg | 产品价格： | 询价 |
Mouse anti Rat CD63 antibody, clone AD1 recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell types in vitro (Nishikata et al. 1992).
Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitani et al. 1991).
Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitani et al. 1991).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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文献和实验该产品被引用文献
Source Reference
- Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.
J Biol Chem. 266 (3): 1903-9.
Antibody Characterization Reference
- Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.
J Immunol. 149(3): 862-70.
References for CD63 antibody
- Boros, M. et al. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat.
Shock. 3 (2): 125-31. - Vincent-Schneider, H. et al. (2001) Secretory granules of mast cells accumulate mature and immature MHC class II molecules
J Cell Sci. 114(Pt 2): 323-34. - Kraft, S. et al. (2005) Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo.
J Exp Med. 201: 385-96. - Ramezani-Moghadam, M. et al. (2015) Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion.
J Biol Chem. 290(9): 5533-42. - Yoshimura, A. et al. (2016) Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids.
Sci Rep. 6: 31172. - Pasquier, A. et al. (2019) Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
Nat Commun. 10 (1): 3312. - Pusic, K.M. et al. (2019) IFNγ-Stimulated Dendritic Cell Exosomes for Treatment of Migraine Modeled Using Spreading Depression.
Front Neurosci. 13: 942.
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Mouse anti Rat CD63:FITC，clone AD1
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