- 询价
- BIO-RAD
- MCA77D680
- 2025年10月17日
- WB
- Yeast
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Yeast tubulin.
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to DyLight®680 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
DyLight®680
- 适应物种：
Yeast
- 保质期：
自发货之日起 12 个月
- 目录编号：
YL1/2
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Tubulin Alpha antibody | YL1/2
- 规格：
0.1 mg
Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).
Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).
Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | ✓ | 1/500 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
- Western Blotting
- MCA77D680 is suitable for use as a loading control
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文献和实验该产品被引用文献
References for Tubulin Alpha antibody
- Kilmartin, J.V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82. - Wehland, J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.
J Cell Biol. 97 (5 Pt 1): 1467-75. - Wehland, J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
EMBO J. 3 (6): 1295-300. - Burns, R. (1987) Cytoskeleton. Tubulin's terminal tyrosine.
Nature. 327 (6118): 103-4. - Skinner, R.H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins.
J Biol Chem. 266 (22): 14163-6. - Berrueta, L. et al. (1998) The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules.
Proc Natl Acad Sci U S A. 95: 10596-601. - Gordon-Weeks, R. et al. (2003) Restricted spatial expression of a high-affinity phosphate transporter in potato roots.
J Cell Sci.116: 3135-44. - Ligon, L.A. et al. (2003) The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.
Mol Biol Cell. 14: 1405-17.
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文献支持
Rat anti Tubulin Alpha:DyLight®680，clone YL1/2
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