- 询价
- BIO-RAD
- MCA77G
- 2025年10月17日
- C, E, IF, IP, R, WB
- Yeast
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Yeast tubulin.
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Yeast
- 保质期：
自发货之日起 12 个月
- 目录编号：
YL1/2
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, IF, IP, R, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Tubulin Alpha antibody | YL1/2
- 规格：
0.5 mg
Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).
Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.
The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).
Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 1/100 | 1/1000 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ | ||
| Radioimmunoassays | ✓ | ||
| Western Blotting | ✓ | 1/200 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rat anti tubulin alpha antibody, clone YL1/2 recognizes a band of ~55 kDa in cell lysate from a wide range of species. Rat anti tubulin alpha antibody, clone YL1/2 is suitable for use as a western blotting loading control.
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文献和实验该产品被引用文献
References for Tubulin Alpha antibody
- Kilmartin, J.V. et al. (1982) Rat monoclonal anti tubulin antibodies derived by using a new nonsecreting rat cell line.
J Cell Biol. 93 (3): 576-82. - Wehland, J. et al. (1983) A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.
J Cell Biol. 97 (5 Pt 1): 1467-75. - Wehland, J. et al. (1984) Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
EMBO J. 3 (6): 1295-300. - Burns, R. (1987) Cytoskeleton. Tubulin's terminal tyrosine.
Nature. 327 (6118): 103-4. - Skinner, R.H. et al. (1991) Use of the Glu-Glu-Phe C-terminal epitope for rapid purification of the catalytic domain of normal and mutant ras GTPase-activating proteins.
J Biol Chem. 266 (22): 14163-6. - Berrueta, L. et al. (1998) The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules.
Proc Natl Acad Sci U S A. 95: 10596-601. - Gordon-Weeks, R. et al. (2003) Restricted spatial expression of a high-affinity phosphate transporter in potato roots.
J Cell Sci.116: 3135-44. - Ligon, L.A. et al. (2003) The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.
Mol Biol Cell. 14: 1405-17.
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Rat anti Tubulin Alpha，clone YL1/2
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