- 询价
- BIO-RAD
- MCA1360D405GA
- 2025年10月17日
- F, IF
- Viral
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Paramyxovirus Simian-Virus 5 (SV5)
- 亚型：
IgG2a
- 形态：
Purified IgG conjugated to DyLight® 405 - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
DyLight®405
- 适应物种：
Viral
- 保质期：
自发货之日起 12 个月
- 目录编号：
SV5-Pk1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F, IF
- 浓度：
IgG concentration 1.0mg/ml
- 抗体名：
V5-Tag antibody | SV5-Pk1
- 规格：
0.1 mg
Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/100 | 1/1000 |
| Immunofluorescence | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献和实验该产品被引用文献
References for V5-Tag antibody
- Southern, J.A. et al. (1991) Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.
J Gen Virol. 72 ( Pt 7): 1551-7. - Randall, R.E. et al. (1994) Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming.
Vaccine. 12 (4): 351-8. - Hirst, K. et al. (1994) The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal.
EMBO J. 13 (22): 5410-20. - Rodriguez, M.S. et al. (1995) Inducible degradation of I kappa B alpha in vitro and in vivo requires the acidic C-terminal domain of the protein.
Mol Cell Biol. 15 (5): 2413-9. - Jaffray, E. et al. (1995) Domain organization of I kappa B alpha and sites of interaction with NF-kappa B p65.
Mol Cell Biol. 15 (4): 2166-72. - Hanke, T. et al. (1995) Attachment of an oligopeptide epitope to the C-terminus of recombinant SIV gp160 facilitates the construction of SMAA complexes while preserving CD4 binding.
J Virol Methods. 53 (1): 149-56. - Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
J Immunol Methods. 224 (1-2): 141-50. - Young, D.F. et al. (2001) Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells.
J Virol. 75: 3363-70.
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文献支持
Mouse anti V5-Tag:DyLight®405，clone SV5-Pk1
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