- 询价
- BIO-RAD
- MCA1396P
- 2025年10月17日
- E, WB
- Synthetic Peptide
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
PAX6 transcription factor linked to histidine tag.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
HRP
- 适应物种：
Synthetic Peptide
- 保质期：
自发货之日起 12 个月
- 目录编号：
AD1.1.10
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Histidine Tag antibody | AD1.1.10
- 规格：
0.1 mg
Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (
Hoffmann
et al. 2007) and
Hwang
et al. 2008) and non-mammalian (
Zheng
et al. 2007;
Gunnarsen
et al. 2010; and
de Vooght et al. 2012) cell lines.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 1/20 | 1/1000 |
| Western Blotting | ✓ | 1/1000 | 1/5000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献和实验该产品被引用文献
References for Histidine Tag antibody
- Els Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs.
J Biol Chem. 276 (10): 7346-50. - Suen, J.L. et al. (2001) Characterization of self-T-cell response and antigenic determinant of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice.
Immunol. 103: 301-309. - Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells.
J Biol Chem. 282 (31): 22370-5. - Zheng, J. et al. (2007) Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen.
BMC Immunol. 8: 4. - Bahi, A. & Dreyer, J.L. (2008) Overexpression of plasminogen activators in the nucleus accumbens enhances *-, amphetamine- and *-induced reward and behavioral sensitization.
Genes Brain Behav. 7 (2): 244-56. - Wrighton, K.H. et al. (2009) Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors.
J Biol Chem. 284 (15): 9755-63. - Diefenbacher, M. et al. (2011) The Dsl1 Tethering Complex Actively Participates in Soluble NSF (N-Ethylmaleimide-sensitive Factor) Attachment Protein Receptor (SNARE) Complex Assembly at the Endoplasmic Reticulum in Saccharomyces cerevisiae.
J Biol Chem. 286: 25027-38. - Alvarez, M.M. et al. (2010) Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method.
PLoS One. 5: e10176.
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文献支持
Mouse anti Histidine Tag:HRP，clone AD1.1.10
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