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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant soluble JAM-C.
- 亚型:
IgG2a
- 形态:
Purified IgG conjugated to Biotin - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Biotin
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
CRAM-19 H36
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, E, F, IF, IP
- 浓度:
IgG concentration 0.1 mg/ml
- 抗体名:
JAM-C antibody | CRAM-19 H36
- 规格:
0.1 mg
Rat anti Mouse JAM-C, clone CRAM-19 H36 recognizes mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.
JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.
JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM-B.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Flow Cytometry | ✓ | Neat | 1/5 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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文献和实验该产品被引用文献
References for JAM-C antibody
- Aurrand-lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells.
J Biol Chem. 276 (4): 2733-41. - Johnson-léger, C.A. et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration.
Blood. 100 (7): 2479-86. - Morita, Y. et al. (2010) Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.
J Exp Med. 207 (6): 1173-82.
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文献支持
Rat anti Mouse JAM-C:Biotin,clone CRAM-19 H36
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