- 询价
- BIO-RAD
- HCA257P
- 2025年10月17日
- E
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Tocilizumab and tocilizumab-sIL-6R complex
- 亚型：
IgG1
- 形态：
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- 克隆性：
Monoclonal Antibody
- 标记物：
HRP
- 保质期：
自发货之日起 12 个月
- 目录编号：
AbD22155_hIgG1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Tocilizumab antibody | AbD22155_hIgG1
- 规格：
0.1 mg
Human Anti-Tocilizumab Antibody, clone AbD22155_hIgG1 is a non-paratope specific, anti-idiotypic antibody that binds to free tocilizumab and also to tocilizumab bound to its target, human interleukin-6 receptor (IL-6R). The non-inhibitory antibody can be used to measure total levels of tocilizumab and biosimilar products in bioanalytical assays.
Clone AbD22155_hIgG1 can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug levels (free drug, partially bound and fully bound). This non-inhibitory antibody is recommended as the detection antibody, paired with the inhibitory capture antibody clone AbD21362 (HCA252). This combination enables measurement of free and partially bound tocilizumab, which is equivalent to total drug when trough serum levels are above 10 μg/ml.
Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.
View a summary of all anti-tocilizumab antibodies
Clone AbD22155_hIgG1 can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug levels (free drug, partially bound and fully bound). This non-inhibitory antibody is recommended as the detection antibody, paired with the inhibitory capture antibody clone AbD21362 (HCA252). This combination enables measurement of free and partially bound tocilizumab, which is equivalent to total drug when trough serum levels are above 10 μg/ml.
Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.
View a summary of all anti-tocilizumab antibodies
储存信息
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
-
This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
References for Tocilizumab antibody
- Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1 - Arceo, T. et al. (2025) Impact of positive control binding properties on anti-drug antibody assay performance.
Bioanalysis. June 12 : 1-6 [Epub ahead of print].
技术资料暂无技术资料 索取技术资料
文献支持
Human anti Tocilizumab:HRP，clone AbD22155_hIgG1
询价
