- 询价
- BIO-RAD
- MCA341B
- 2025年10月17日
- C
- Rat
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Rat spleen cells.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Biotin - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin
- 适应物种：
Rat
- 保质期：
自发货之日起 12 个月
- 目录编号：
ED1
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C
- 浓度：
IgG concentration 0.1 mg/ml
- 抗体名：
CD68 antibody | ED1
- 规格：
100 Tests
Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).
The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).
The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).
The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | ✓ | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.antibodies.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
Source Reference
- Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99.
References for CD68 antibody
- Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1.
Immunology. 83 (1): 140-7. - Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis.
J Neurosci Res. 38 (4): 365-75. - Kornek, B. et al. (2000) Multiple sclerosis and chronic autoimmune encephalomyelitis: a comparative quantitative study of axonal injury in active, inactive, and remyelinated lesions.
Am J Pathol. 157: 267-76. - Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats.
J Neurochem. 88 (6): 1335-44. - Thom, S.R. et al. (2004) Delayed neuropathology after carbon monoxide poisoning is immune-mediated.
Proc Natl Acad Sci U S A. 101 (37): 13660-5. - Wu, L. et al. (2004) Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system.
Proc Natl Acad Sci U S A. 101 (18): 7094-9. - Pellkofer, H. et al. (2004) Modelling paraneoplastic CNS disease: T-cells specific for the onconeuronal antigen PNMA1 mediate autoimmune encephalomyelitis in the rat.
Brain. 127 (Pt 8): 1822-30. - Korn, T. et al. (2004) Modulation of effector cell functions in experimental autoimmune encephalomyelitis by leflunomide--mechanisms independent of pyrimidine depletion.
J Leukoc Biol. 76 (5): 950-60.
技术资料暂无技术资料 索取技术资料
文献支持
Mouse anti Rat CD68:Biotin，clone ED1
询价
