- 询价
- BIO-RAD
- MCA6048GA
- 2025年10月17日
- F
- Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Porcine lymphocytes
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Pig
- 保质期：
自发货之日起 12 个月
- 目录编号：
11/295/33
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
CD8 Alpha antibody | 11/295/33
- 规格：
0.1 mg
Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.
In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).
In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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文献和实验该产品被引用文献
References for CD8 Alpha antibody
- Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
Vaccines. 10 (5): 684.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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文献支持
Mouse anti Pig CD8 Alpha，clone 11/295/33
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