APOBEC3B Rabbit pAb(bs-12494R)-50ul/100ul/200ul
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APOBEC3B Rabbit pAb(bs-12494R)

-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-12494R
  • 2025年10月17日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-12494R
    英文名称APOBEC3B Rabbit pAb
    中文名称载脂蛋白B-mRNA编辑酶复合物3B抗体
    英文别名APOBEC1L; Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B; apolipoprotein B mRNA editing enzyme, catalytic; ARCD3; ARP4; bK150C2.2; Cytidine deaminase; DJ742C19.2; FLJ21201; Phorbolin 1 related protein; Phorbolin 2; Phorbolin 2/3; Phorbolin 3; PHRBNL; Probable DNA dC>dU editing enzyme APOBEC3B.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat, Mouse
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human APOBEC3B
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量46 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Deamination

    Immunology > Immune System Diseases > Antiviral Signaling

    亚基Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1.
    亚细胞定位Nucleus.
    组织特异性Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues.
    相似性It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22.
    Belongs to the cytidine and deoxycytidylate deaminase family.
    Contains 2 CMP/dCMP deaminase zinc-binding domains
    功能APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料APOBEC proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. APOBEC3 proteins are thought to be RNA editing enzymes and have roles in cell growth. APOBEC3B, also known as phorbolin-1-related protein or phorbolin-2/3, is a 382 amino acid protein belonging to the cytidine and deoxycytidylate deaminase family. APOBEC3B lacks cytidine deaminase activity on RNA molecules, but has been shown to bind to apoB and AU-rich RNAs. APOBEC3B forms a homodimer and interacts with APOBEC3G. APOBEC3B is expressed at at moderate levels in heart, testis, thymus, prostate, ovary, spleen and peripheral blood leukocytes.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    APOBEC3B Rabbit pAb(bs-12494R)
    Tissue/cell: rat spleen tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    APOBEC3B Rabbit pAb(bs-12494R)
    Tissue/cell: Rat ovary tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    APOBEC3B Rabbit pAb(bs-12494R)
    Tissue/cell: Mouse spleen tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    APOBEC3B Rabbit pAb(bs-12494R)
    Sample:jurkat(human)cell Lysate at 40 ug
    Primary: Anti-APOBEC3B (bs-12494R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 46kD
    Observed band size: 46 kD

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    图标文献和实验
    该产品被引用文献

    [IF={{ 2.02 }}] {Onguru, Onder, et al. "APOBEC3B expression in drug resistant MCF-7 breast cancer cell lines." Biomedicine & Pharmacotherapy 79 (2016): 87-92.} {WB,IHC} {="Human"}

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    文献支持
    APOBEC3B Rabbit pAb(bs-12494R)-50ul/100ul/200ul
    ￥1180 - 2800