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- bs-13261R
- 2025年10月17日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13261R |
| 英文名称 | GABPA Rabbit pAb |
| 中文名称 | GA结合蛋白转录因子α/GABP-α抗体 |
| 英文别名 | E4TF 1A; E4TF1 60; E4TF1A; GA binding protein alpha chain; GA binding protein transcription factor alpha subunit 60kDa; GA binding protein transcription factor alpha subunit; GA-binding protein alpha chain; GABP A; GABP alpha subunit; GABP alpha subunit; GABP subunit alpha; Gabpa; GABPA_HUMAN; Human nuclear respiratory factor 2 subunit alpha; NFT 2; NFT2; NRF 2; NRF 2A; NRF2; NRF2A; Nuclear respiratory factor 2 alpha subunit; Nuclear respiratory factor 2 subunit alpha; Nuclear respiratory factor 2 subunit alpha; RCH04A07; Transcription factor E4TF1 60; Transcription factor E4TF1 60; Transcription factor E4TF1-60. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Chicken, Dog, Pig, Cow, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GABPA |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚基 | Heterotetramer of two alpha and two beta subunits. |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain. |
| 功能 | Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-alpha and a GABP-alpha-associated subunit, GABP beta. GABP alpha binds to a specific DNA sequence and GABP beta exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP beta is expressed in both the cytoplasm and the nucleus, whereas GABP alpha is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABP alpha beta heterodimer or a GABP alpha b heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo. The GABP alpha beta tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated (bs-13261R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- GABPA (bs-13261R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
293T Cell (Human) Lysate at 30 ug
Primary: Anti- GABPA (bs-13261R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
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[IF={{ 3.738 }}] {Rao, Gan. et al. Arsenic Trioxide Triggers Mitochondrial Dysfunction, Oxidative Stress, and Apoptosis via Nrf 2/Caspase 3 Signaling Pathway in Heart of Ducks. BIOL TRACE ELEM RES. Biol Trace Elem Res. 2022 Apr;:1-11} {WB} {Duck}
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GABPA Rabbit pAb(bs-13261R)-50ul/100ul/200ul
￥1180 - 2800
