Blank control:ctll-2.
Primary Antibody (green line): Rabbit Anti-mouse CD25 antibody (bsm-30157A)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-rat IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rat IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1880
- Bioss
- bsm-30157A
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ug
| 产品编号 | bsm-30157A |
| 英文名称 | mouse CD25 Rat mAb |
| 中文名称 | 小鼠CD25单克隆抗体 |
| 英文别名 | Interleukin 2 receptor alpha chain; CD 25; CD25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL2 RA; IL-2RA; IL2R; IL2RA; IL2RAC; Interleukin 2 Receptor alpha; Interleukin 2 receptor alpha chain; Interleukin 2 receptor; Ly43; p55; T Cell Growth Factor Receptor; TAC Antigen; TCGFR; IL2RA_MOUSE. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Mouse |
| 抗体来源 | Rat |
| 亚型 | Rat IgG1, λ |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 28 kDa |
| 实际分子量 | 55kDa |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > T Cells > CD Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚基 | Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 相似性 | Contains 2 Sushi (CCP/SCR) domains. |
| 功能 | Receptor for interleukin-2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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mouse CD25 Rat mAb(bsm-30157A)-100ug
￥1880
