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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-33206R |
| 英文名称 | S1-Tag Rabbit pAb |
| 中文名称 | S1标签抗体 |
| 英文别名 | S1 Tag; S1Tag; NANNPDWDF; |
| 产品应用 | WB=1:1000-5000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide S1-Tag |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged protein's biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {ELISA} | {1:1000-5000} |

Lane 1: S1-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: S1-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-S1-Tag (bs-33206R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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文献和实验干货 | CUT and Tag 让 ChIP-Seq更简单高效!
说明书推荐的反应体系进行 CUT&Tag 实验;其中,pG-Tn5 转座子使用的终浓度为 0.04μM ,一抗为 H3K27me3(CST,#9733),二抗为 Goat anti Rabbit(Bioworld,#BS13271)。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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of rabbit polyclonal antibodies directed to the major sodium transporters and water channels expressed in each renal tubule segment.” It allows and facilitates characterization and analysis of tubular functional differences that define individual nephron
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