Blank control:TF-1.
Primary Antibody (green line): Mouse Anti-human CD34 antibody (bsm-30097M)
Dilution: 0.5ug/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Mouse IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1880
- Bioss
- bsm-30097M
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ug
| 产品编号 | bsm-30097M |
| 英文名称 | human CD34 Mouse mAb |
| 中文名称 | 小鼠抗人CD34单克隆抗体 |
| 英文别名 | CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN. |
| 产品应用 | Flow-Cyt=0.5ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, placenta, placenta, placenta, placenta |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD34 |
| 亚型 | Mouse IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 39 kDa |
| 实际分子量 | 105-120kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4). |
| 研究领域 | Cancer > Tumor biomarkers Cardiovascular > Angiogenesis > Endothelial Cell Markers Cardiovascular > Heart > Cardiogenesis > Stem cells Developmental Biology > Lineage specification > Mesoderm Immunology > Cell Type Markers > CD > Myeloid Cells Neuroscience > Cell Type Marker > Neuron marker > Soma marker Stem Cells > Endothelial Progenitors > Endothelial Markers Stem Cells > Hematopoietic Progenitors > Hematopoietic Stem Cells > HSC markers Stem Cells > Hematopoietic Progenitors > Surface Molecules Stem Cells > Lineage Markers > Mesoderm Stem Cells > Mesenchymal Stem Cells > Negative Markers |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. |
| 翻译后修饰 | Highly glycosylated. Phosphorylated on serine residues by PKC. |
| 相似性 | Belongs to the CD34 family. |
| 功能 | Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {0.5ug/Test} |
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human CD34 Mouse mAb(bsm-30097M)-100ug
￥1880
