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GAP43 Rabbit pAb(bs-0154R)-50u

l/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-0154R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0154R
    英文名称GAP43 Rabbit pAb
    中文名称神经生长相关蛋白43抗体
    英文别名Growth Associated Protein-43; Neuromodulin; Axonal membrane protein GAP 43; B-50; F1; GAP 43; Growth Associated Protein 43; Nerve Growth Related Peptide; Neural phosphoprotein B 50; Neuromodulin; GAP-43; pp46; NEUM_HUMAN; Protein F1; QtrA-11580; QtrA-13071.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Human Glioma, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Cerebrum (Chicken, Dog)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GAP43
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量25/46 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Type Marker > Neuron marker > Growth Cone

    Neuroscience > Cell Type Marker > Neuron marker > Synapse marker

    Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins

    亚基Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.
    亚细胞定位Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
    翻译后修饰Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
    Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
    相似性Belongs to the neuromodulin family.
    Contains 1 IQ domain.
    功能This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:200-800}

     

    产品细节图片1
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-GAP-43 Polyclonal Antibody, Unconjugated(bs-0154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAP43) Polyclonal Antibody, Unconjugated (bs-0154R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 5.546 }}] {Yifan Wang. et al. Positive effect of Astragaloside IV on neurite outgrowth via talin‐dependent integrin signaling and microfilament force. J Cell Physiol. 2021 Mar;236(3):2156-2168} {IF} {Human}

    [IF={{ 4.73 }}] {Erdal Ayhan Işik. et al. Use of Erythropoietin and Fibrin Glue Mixture for Peripheral Nerve Repair. Plast Reconstr Surg. 2022 Feb;149(2):395-403} {IHC} {Rat}

    [IF={{ 4.6 }}] {Yumei Zheng. et al.Abdominal LIPUS Stimulation Prevents Cognitive Decline in Hind Limb Unloaded Mice by Regulating Gut Microbiota.MOLECULAR NEUROBIOLOGY.2025 Jan 29.} {Western blot} {Mouse}

    [IF={{ 4.411 }}] {Pingan Chen. et al. The influence of inhibiting renal neural regeneration on the efficacy of renal denervation to chronic heart failure. 2021 Oct 22} {WB} {Rat}

    [IF={{ 3.297 }}] {Sheng Cao. et al. Ultrasound-targeted microbubble destruction-mediated Ang1 gene transfection improves left ventricular structural and sympathetic nerve remodeling in canines with myocardial infarction. Ann Transl Med. 2021 Feb; 9(3): 221} {IHC} {Dog}

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    GAP43 Rabbit pAb(bs-0154R)-50ul/100ul/200ul
    ￥1180 - 2800