Sample:
Lane1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Lane2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4ug
Primary: Anti- Myc tag (bs-23166R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 43 kD
- ￥980 - 4000
- Bioss
- bs-23166R
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul/500ul
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 产品编号 | bs-23166R |
| 英文名称 | Myc tag Rabbit pAb |
| 中文名称 | Myc tag标签抗体 |
| 英文别名 | Avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c Myc Epitope Tag; c Myc Tag; c Myc-Tag; C-Myc-Tag; Cellular myelocytomatosis oncogene; MGC105490; MRTL; Myc Epitope Tag; Myc protein; Myc proto oncogene protein; Myc proto-oncogene protein; Myc-related translation/localization regulatory factor; Myc2; myca; Myelocytomatosis oncogene a; Myelocytomatosis oncogene; Niard; Nird; Oncogene Myc; Protooncogene homologous to myelocytomatosis virus; RNCMYC; Transcription factor p64; Transcriptional regulator Myc-A; V-Myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog |
| 产品应用 | WB=1:1000-10000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Myc tag(EQKLISEEDL) |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell differentiation Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors Cancer > Tumor biomarkers > Oncoproteins Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH / Leucine Zipper Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Stem Cells > Signaling Pathways > TGF beta > Nuclear Tags & Cell Markers > Epitope Tags > Myc Tag |
| 亚基 | Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2 (By similarity). Interacts with NO66. |
| 亚细胞定位 | Nucleus. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Epitope tags are useful for the labeling and detection of proteins usingimmunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-10000} |
| {ELISA} | {1:1000-5000} |
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文献和实验该产品被引用文献
[IF={{ 4.8 }}] {Mingjiao Zhang. et al. TRIM26 restricts Epstein–Barr virus infection in nasopharyngeal epithelial cells through K48-linked ubiquitination of HSP-90β. FASEB J. 2023 Dec;38(1):e23345} {WB,CoIP} {Human}
[IF={{ 4.7 }}] {Ming Luo. et al. Fecal Microbiota Transplantation Alleviates Cirrhotic Portal Hypertension in Rats via Butyrate-Mediated HDAC3 Inhibition and PI3K/Akt/eNOS Signaling Regulation. EUR J PHARMACOL. 2025 May;:177781} {CoIP} {Rat}
[IF={{ 21.1 }}] {Xie Man-Xiu. et al. Endophilin A2 controls touch and mechanical allodynia via kinesin-mediated Piezo2 trafficking. MILITARY MED RES. 2024 Dec;11(1):1-27} {WB,CoIP} {Mouse}
[IF={{ 17.521 }}] {Han Liu. et al. Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation. Advanced Science. 2022 Sep;:2203995} {IF} {Human}
相关实验
Studies of the Ubiquitin Proteasome System
with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation
that can lead to artifacts. The advantage to using exogenously expressed tagged proteins is the generally high specificity of the anti-tag antibody and the ease with which a negative control can be performed in which the exogenous protein is lacking.
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Myc tag Rabbit pAb(bs-23166R)-100ul/500ul
￥980 - 4000
