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- 2025年10月16日
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500ug
| 产品编号 | bs-9724P |
| 英文名称 | E4F1 Antibody Blocking Peptide |
| 中文名称 | E4F转录因子1封闭多肽 |
| 英文别名 | E4F; E4F transcription factor 1, isoform CRA_b; P120E4F transcription factor; E4F1_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Cell Biology > Cell Cycle > Cell differentiation Epigenetics and Nuclear Signaling > Transcription > Transcription Factors |
| 亚基 | Homodimer; binds DNA as a dimer. Forms a complex with CDKN2A and TP53. Interactions with TP53, RB1, ANP32A, BMI1 and FHL2 regulate E4F1 activity. Interacts with HDAC1, HMGA2 and RASSF1. Interacts with HBV protein X. |
| 亚细胞定位 | Nucleus, nucleoplasm. Cytoplasm. Note=A small fraction is detected in the cytoplasm. Excluded from the nucleolus where it is targeted upon CDKN2A overexpression. Localizes to the mitotic spindle during embryogenesis (By similarity). |
| 组织特异性 | Ubiquitously expressed. |
| 翻译后修饰 | Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full length protein (p120E4F) functions as a repressor of transcription. Phosphorylated; p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell cycle-dependent and differentially regulates DNA-binding activity and function of both forms. May be sumoylated by UBE2I upon interaction with CDKN2A. |
| 相似性 | Contains 9 C2H2-type zinc fingers. |
| 功能 | May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription. [FUNCTION] Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | E4F1, also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains nine C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities. |
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文献和实验or from the literature are missing. In this article, processing parameters for DNA, peptide, antibody, and carbohydrate microarrays are outlined. The applicability of the model experiments is demonstrated and described in detail on the example of short oligonucleotides.
Synthesis and Probing of Membrane-bound Peptide Arrays
the stringency of the blocking conditions and make sure that the primary binding partner and detection reagent (e.g., antibody) are of high purity and are used in the highest possible dilution. Stage
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
. Fack, F., Deroo, S., Kreis, S., and Muller, C.P. 2000. Heteroduplex mobility assay (HMA) pre‐screening: An improved strategy for the rapid identification of inserts selected from phage‐displayed peptide
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