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500ug
| 产品编号 | bs-20664P |
| 英文名称 | phospho-Fos B (Ser27) Antibody Blocking Peptide |
| 中文名称 | 磷酸化癌基因FOS蛋白B封闭多肽 |
| 英文别名 | Fos B (phospho S27); p-Fos B (phospho S27); p-FosB (phospho S27); FBJ murine osteosarcoma viral oncogene homolog B; FOS B; FOS-B; FBJ osteosarcoma oncogene B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB; fosB; G0/G1 switch regulatory protein 3; G0S3; GOS 3; GOS B; GOSB; Oncogene FOS B; Oncogene FOSB; Protein fos B; DKFZp686C0818; FOSB_HUMAN. |
| 纯化方法 | HPLC |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Proto-oncogenes Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > Leucine Zipper |
| 亚基 | Heterodimer. |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. |
| 功能 | FosB interacts with Jun proteins enhancing their DNA binding activity. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, Fos B, (fos-related antigen) Fra 1, and Fra 2. Also named as DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FosB; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; Oncogene FOS B; Protein fosB. |
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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
the stringency of the blocking conditions and make sure that the primary binding partner and detection reagent (e.g., antibody) are of high purity and are used in the highest possible dilution. Stage
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Adey, N.B., Mataragonon, A.H., Rider, J.E., Carter, J.M., and Kay, B.K. 1995. Characterization of phage that bind plastic from phage‐displayed random peptide libraries. Gene 156:27‐31
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
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