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- 2025年10月16日
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| 规格： | 100ug | 产品价格： | ￥1880.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥5680.0 |
| 规格： | 20ug | 产品价格： | ￥580.0 |
| 产品编号 | bs-2194P |
| 英文名称 | Recombinant EGFP protein, GST |
| 中文名称 | 重组增强型绿色荧光蛋白 |
| 英文别名 | Enhanced Green Fluorecent protein; rEGFP; Recombinant EGFP; Enhanced Green Fluorecent protein; Recombinant EGFP protein, GST |
| 性状 | Lyophilized or Liqui |
| 纯化方法 | AC |
| 理论分子量 | 27 |
| 实际分子量 | 55 kDa |
| 储存液 | Lyophilized powder (Lyophilized from 0.22um filtered solution in 10mM TBS (pH=7.4). Normally 5% trehalose is added as protectant before lyophilization.). |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > GFP |
| 亚基 | Monomer. |
| 组织特异性 | Photocytes. |
| 翻译后修饰 | Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. |
| 相似性 | Belongs to the GFP family. |
| 功能 | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enhanced green fluorescence protein (EGFP) is a 27 kDa protein derived fromthe jellyfish Aequorea victoria, which emits green light (emission peak at awavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenthof 395 nm). EGFP has become an invaluable tool in cell biology research, sinceits intrinsic fluorescence can be visualized in living cells. EGFP fluorescence isstable under fixation conditions and suitable for a variety of applications. EGFPhas been widely used as a reporter for gene expression, enabling researchers tovisualize and localize EGFP-tagged proteins within living cells without the needfor chemical staining. Other applications of EGFP include assessment of proteinprotein interactions through the yeast two hybrid system and measurement ofdistance between proteins through fluorescence energy transfer (FRET)protocols. EGFP technnology has considerably contributed to a greaterunderstanding of cellular physiology. |
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible
Recombinant antigens for immunodiagnosis of cystic echinococcosis
) and with sera from mice infected with E. granulosus oncospheres. The cDNAs were subcloned into a pET vector, expressed as fusion proteins tagged with GST and affinity purified against the GST tag. Of the three recombinant proteins, EpC1 achieved the highest
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
‐throughput protein expression screening. Genomics 65:1‐8. Cheung, K.H., Hager, J., Nelson, K., White, K., Li, Y., Snyder, M., Williams, K., and Miller, P. 2002. A dynamic approach
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