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500ug
| 产品编号 | bs-3263P |
| 英文名称 | Phospho-MARCKS (Ser152/156) (Ser159/163 hu) Antibody Blocking Peptide |
| 中文名称 | 磷酸化丙氨酸蛋白激酶C底物Marcks封闭多肽 |
| 英文别名 | MARCKS (phospho S152 + S156); p-MARCKS (phospho S152 + S156); MARCKS (phospho S159 + S163); 80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_RAT; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate. |
| 纯化方法 | HPLC |
| 研究领域 | Cardiovascular > Vasculature > Endothelium Cardiovascular > Vasculature > Smooth muscle cell (SMC) > Cytoskeleton Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > PKC Signal Transduction > Signaling Pathway > Calcium Signaling > Calmodulin Pathway |
| 亚细胞定位 | Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor. |
| 组织特异性 | Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. |
| 翻译后修饰 | Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. |
| 相似性 | Belongs to the MARCKS family. |
| 功能 | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| 保存条件 | Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin. |
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文献和实验secondary antibody review -- data from 99 publications
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Method Determine the highest antibody dilution at which the optimal working dilution is used with the antibody in Western blotting. For neutralization, combine antibody with a fivefold (by weight) excess of blocking peptide in a small
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4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
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