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- bs-49058P
- 2025年10月16日
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100ug/500ug/20ug
| 规格： | 100ug | 产品价格： | ￥1880.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥5680.0 |
| 规格： | 20ug | 产品价格： | ￥580.0 |
| 产品编号 | bs-49058P |
| 英文名称 | Recombinant H5N6-NA protein, His |
| 中文名称 | 重组H5N6-NA蛋白 |
| 英文别名 | A0A240EUK5_9INFA; Neuraminidase · Influenza A virus (A/oriental magpie robin/HK/6154/2015(H5N6)); Neuraminidase; NA; GenBank: AOC37464.1; Recombinant H5N6-NA protein, His |
| 性状 | Lyophilized or Liqui |
| 纯化方法 | AC |
| 理论分子量 | 50 |
| 实际分子量 | 55 kDa |
| 浓度 | ＞0.5 mg/ml |
| 储存液 | 20mM Tris-HCl (pH8.0) with 150mM NaCl. |
| 保存条件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication. |
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文献和实验DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
George P.Smith Division of Biological Sciences Tucker Hall (573)882-3344 Introduction to B-PER B-PER from Pierce Chemical Co.is a Tris-buffered detergent formulation that lyses bacterial cells without denaturing enzymes or other bioactive
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
of these samples, and this is particularly true for the peripheral membrane extract (see note above). All frozen aliquots must be centrifuged after defrosting at 35,000 × g (or maximum speed) for 30 min at 4°C, before incubation with recombinant GST fusion protein
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