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- IHC0635M
- 2025年10月15日
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50T
| 产品编号 | IHC0635M |
| 英文名称 | Mouse ALYREF Ready-To-Use IHC Kit |
| 中文名称 | 小鼠ALYREF即用型免疫组化试剂盒 |
| 英文别名 | ALY; ALY/REF; ALYREF; alyref-a; BEF; REF; Tho4-A; THOC4; thoc4-a; XELAEV_18043750mg |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Mouse |
| 亚基 | Homomultimer; predominantly hexamer when bound to EJC-RNA complex (PubMed:16314458, PubMed:37020021). Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex (THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7); in the complex interacts (via UAP56-binding motif) with DDX39B with low affinity but this interaction is likely stabilized by multimerization (PubMed:11979277, PubMed:12944400, PubMed:15833825, PubMed:15998806, PubMed:17190602, PubMed:17984224, PubMed:18974867, PubMed:23826332, PubMed:37020021). TREX seems to have a dynamic structure involving ATP-dependent remodeling; in the complex interacts with THOC1, THOC2 and THOC5 (PubMed:19165146, PubMed:23222130, PubMed:37020021). Component of the ALYREF/THOC4-EJC-RNA complex; in the complex interacts (via the WXHD motif) with EIF4A3 and interacts (via the RRM domain) with MAGOH; these interactions are likely specific to RNA-bound EJC (PubMed:14730019, PubMed:16314458, PubMed:37020021). Bridges the THO-DDX39B and EJC-RNA complexes to form the TREX-EJC-RNA complex; this interaction is essential for mRNP recognition and mRNA packaging (PubMed:37020021). Identified in the spliceosome C complex (PubMed:11991638, PubMed:12176931). Found in a mRNP complex with UPF3A and UPF3B (PubMed:11546873). Interacts with RBM8A, RBM15B, NCBP1, LEF1, RUNX1, RNPS1, SRRM1, IWS1 and EXOSC1 (PubMed:11118221, PubMed:11707413, PubMed:12944400, PubMed:17190602, PubMed:17234882). Interacts with RBM15B (PubMed:19586903). Interacts with NXF1; the interaction is direct (PubMed:11675789, PubMed:11707413, PubMed:14730019, PubMed:18974867, PubMed:19165146, PubMed:19586903, PubMed:23826332). Interacts with IVNS1ABP (via BACK domain); the interaction is indirect and likely plays a role in mRNA nuclear export (PubMed:30538201). (Microbial infection) Interacts with human Kaposi's sarcoma-associated herpesvirus (HHV-8) ORF57 protein; this interaction allows efficient export of HHV-8 early and late intronless transcripts. (Microbial infection) Interacts with HHV-1 ICP27 protein; this interaction recruits ALYREF to viral replication compartments and probably directs viral mRNA to the TAP/NXF1 pathway. |
| 亚细胞定位 | #Cytoplasm #Nucleus #Spliceosome |
| 组织特异性 | Expressed in a wide variety of cancer types. |
| 翻译后修饰 | Arg-204 is dimethylated, probably to asymmetric dimethylarginine. Arginine methylation reduces RNA binding.2 Publications Citrullinated by PADI4. |
| 相似性 | Belongs to the ALYREF family. |
| 功能 | Functions as an mRNA export adapter; component of the transcription/export (TREX) complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B (PubMed:17984224). Plays a key role in mRNP recognition and mRNA packaging by bridging the mRNP-bound EJC and the TREX core complex (PubMed:37020021). TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1 (PubMed:15833825, PubMed:15998806, PubMed:17190602, PubMed:37020021). Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC) (PubMed:15998806, PubMed:17984224, PubMed:37020021). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway) (PubMed:11675789, PubMed:11707413, PubMed:11979277, PubMed:15833825, PubMed:15998806, PubMed:17190602, PubMed:18364396, PubMed:22144908, PubMed:22893130, PubMed:23222130, PubMed:25662211). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim (PubMed:19165146). Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling (PubMed:28418038). Acts as a chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation (PubMed:10488337). Involved in transcription elongation and genome stability (PubMed:12438613). (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. |
| 应用 | 推荐稀释比例 |
Immunohistochemical analysis of paraffin embedded Mouse brain tissue slide using IHC0635M (Mouse ALYREF Kit).
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Mouse ALYREF Ready-To-Use IHC Kit(IHC0635M)-50T
￥3200
