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- IHC0451
- 2026年10月13日
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| 产品编号 | IHC0451 |
| 英文名称 | COMT Ready-To-Use IHC Kit |
| 中文名称 | 儿茶酚O甲基移位酶即用型免疫组化试剂盒 |
| 英文别名 | Comt1; D16Wsu103e; D330014B15Rik; HEL-S-98n; Catechol O methyltransferase; Catechol O-methyltransferase; COMT; COMT_HUMAN; EC 2.1.1.6 |
| 产品应用 | IHC-P Not yet tested in other applications. |
| 交叉反应 | Human, Rat |
| 亚细胞定位 | #Cell membrane #Cytoplasm #Membrane |
| 组织特异性 | Brain, liver, placenta, lymphocytes and erythrocytes. |
| 翻译后修饰 | The N-terminus is blocked. |
| 相似性 | Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family. |
| 功能 | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-Methyl DOPA and isoproterenol. |
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 背景资料 | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. |
| 应用 | 推荐稀释比例 |
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文献和实验New double-positivity tool for multi-color IHC IF
by and reflected in local microenvironments, as well as how diseased cells respond to therapies. These kinds of questions can be answered in a limited way by methods that disaggregate or homogenize tissue, or that utilize conventional single-stain IHC
Imaging Techniques in Signal Transduction IHC
values within the dynamic range of the detector, frame averaging to eliminate noise (typically in fluorescence imaging), use of the highest bit depth when a choice is available, flatfield correction, and archiving of the image in a nonlossy format
的染色强度。获得合适的染色强度后，将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体：Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本：石蜡包埋的人类乳腺肿瘤显色检测：利用 SignalStain® DAB Substrate Kit #8059（左），竞争者提供的 DAB（中和右）CST 的 IHC/IF 明星产品推荐：生化试剂：Catalog NumberProduct NameProduct
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