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5g/10g
| 规格: | 5g | 产品价格: | ¥156.0 |
|---|---|---|---|
| 规格: | 10g | 产品价格: | ¥203.0 |
| 产品编号 | D53139 |
| 英文名称 | Disodium monofluorophosphate |
| 中文名称 | 单氟磷酸钠 |
| 英文别名 | Sodium Monofluorophosphate; NSC248 |
| 理论分子量 | 143.95 |
| 保存条件 | Powder: -20℃ for 3 years | In solvent: -80℃ for 1 year |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sodium Monofluorophosphate is a competitive inhibitor of pyruvate kinase and alkaline phosphatase, which also irreversibly inhibits phosphorylase phosphatase. |
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文献和实验("buffer D"): 4M Guanidine thiocyanate, 30 mM disodium citrate, 30 mM β-mercaptoethanol, pH 7.0-7.5 Note: Normally the dispersion buffer does not require titration. If the pH is significantly lower than 7.0, try another batch of guanindine
Human Placental Alkaline Phosphatase Stain
off staining solution with water. STOCK SOLUTIONS XPHOS - 100X solution is 10 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, disodium salt (Sigma) in water, store at -20°C. NBT - 100X solution is 50 mg/ml Nitro Blue tetrazolium (Sigma) in 70
Agarose Gel Electrophoresis of DNA
may be cast with ethidium bromide solution added. This will save time staining but the gel will also run slightly slower (note that ethidium bromide is a powerful mutagen, so be careful in its use). d) In general the supercoiled circular plasmid run faster
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