- ￥2280 - 6480
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- bs-47537P
- 2026年10月10日
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- 文献和实验
- 技术资料
- 规格：
20 μg/50 μg/100 μg
| 规格： | 20 μg | 产品价格： | ￥2280.0 |
|---|---|---|---|
| 规格： | 50 μg | 产品价格： | ￥4680.0 |
| 规格： | 100 μg | 产品价格： | ￥6480.0 |
| 产品编号 | bs-47537P |
| 英文名称 | Recombinant human ADM2 Protein,C-hFc & C-His (HEK293) |
| 中文名称 | 重组人 ADM2 蛋白 |
| 英文别名 | Intermedin |
| 性状 | Liquid |
| 纯化方法 | AC |
| 储存液 | 150 mM PBS |
| 保存条件 | Stored at -70℃ or -20℃ for one year. Avoid repeated freeze/thaw cycles.Stored at 2 to 8 °C for 2 days after thawing. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验Protein detection onto PVDF membranes
in PBS-Tween 0.5% for 30 minutes, washed 3 x 5 min. in PBS-Tween 0.5% and 1 min. in deionized water. They were stained in 100 ml of Problot solution overnight. Ponceau S After electrotransfer, the PVDF membranes were stained in a solution
)9 Incubate 30 min at 37°C.10 Rinse 3x with 0.05% Tween 80 in 0.01 M PBS, pH 7.4.11 Rinse 3x with HPLC water.12 Add 50 µl of color development reagent(1 tablet of p-nitrophenyl reagent in 5 ml of 10% diethanolamine, pH 9.8) (mix well)13 Incubate 30 min at 37°C
Bacterial Protein Extraction (mini-scale)
in 300µl of B-Per reagent (Pierce)by either vigorous vortexing or by pipetting up and down until the cell suspension is homogenous.Vortex for 1 more min.(if suspension is too viscous add Dnase 100U/ml or 25-50µg/ml (SIGMA DN-25).Incubate 10min 4℃
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