Alkaline Phosphatase, Tissue N
on-Specific isozyme Rabbit pAb, Biotin conjugated(bs-1535R-Bio)-100ul- ￥2980
- Bioss
- bs-1535R-Bio
- 2025年09月30日
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100ul
| 产品编号 | bs-1535R-Bio |
| 英文名称 | Alkaline Phosphatase, Tissue Non-Specific isozyme Rabbit pAb, Biotin conjugated |
| 中文名称 | Biotin标记的碱性磷酸酶(组织非特异性)抗体 |
| 英文别名 | AKP2; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme; Alkaline phosphatase tissue nonspecific isozyme; Alkaline phosphatase, tissue-nonspecific isozyme; ALPL; AP TNAP; AP-TNAP; APTNAP; BALP; BAP; FLJ40094; FLJ93059; Glycerophosphatase; HOPS; Liver/bone/kidney isozyme; Liver/bone/kidney type alkaline phosphatase; MGC161443; MGC167935; PHOA; PPBT_HUMAN; Tissue non specific alkaline phosphatase; Tissue nonspecific ALP; TNAP; TNSALP. |
| 中文别名 | 碱性磷酸酶，组织非特异性同工酶; 组织非特异性碱性磷酸酶; 碱性磷酸酶,组织非特异性; |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Alkaline Phosphatase, Tissue Non-Specific isozyme |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homodimer. |
| 亚细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 翻译后修饰 | Glycosylated. |
| 相似性 | Belongs to the alkaline phosphatase family. |
| 功能 | This isozyme may play a role in skeletal mineralization. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase-conjugated antibodies. However, by carefully controlling the reaction time in X-gal so that only a small amount of indigo is produced (Vaysse and Goldman, 1990), by having ß-gal localized to the nucleus when the cellular antigen is non-nuclear
Blocking of unwanted non-specific staining
that may affect your staining. Blocking of endogenous Alkaline Phosphatase is done by exploiting the differential sensitivity of calf intestinal AP, used as a reporter, and leukocyte AP. This latter is inhibited, while the former is not by Levamisole 1 mM
Western Blotting with Alkaline Phosphatase Conjugates
buffer (1% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Incubate the blot for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C. INCUBATION WITH ALKALINE PHOSPHATASE CONJUGATED ANTIBODIES Dilute the AP
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