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- bs-23615R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-23615R-HRP |
| 英文名称 | CD27 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的CD27抗体 |
| 英文别名 | CD 27; CD27 antigen; CD27 molecule; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD27 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > CD markers Cancer > Tumor immunology > Cytokines > TNF Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Immunology > Innate Immunity > Cytokines > TNF Superfamily Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚基 | Homodimer. Interacts with SIVA1 and TRAF2. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Found in most T-lymphocytes. |
| 翻译后修饰 | Phosphorylated. _x000D_ O-glycosylated with core 1 or possibly core 8 glycans. |
| 相似性 | Contains 3 TNFR-Cys repeats. |
| 功能 | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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