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- bs-0437R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-0437R-HRP |
| 英文名称 | Streptavidin Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的链酶亲和素抗体 |
| 英文别名 | SA protein; SA V1; SA V2; Streptavidin V1; Streptavidin V2; SAV1_STRVL. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | Streptavidin protein |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Homotetramer (By similarity). |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the avidin/streptavidin family._x000D_
Contains 1 avidin-like domain. |
| 功能 | The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验[IF={{ 9.7 }}] {Tao Wang. et al. Injectable decellularized extracellular matrix hydrogel promotes salivary gland regeneration via endogenous stem cell recruitment and suppression of fibrogenesis. ACTA BIOMATER. 2023 Aug;:} {IF} {Rat}
[IF={{ 5.7 }}] {Adeel Khan. et al.Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles.FRONTIERS IN IMMUNOLOGY.2025 Jan 23:15:1479403.} {} {}
[IF={{ 4.667 }}] {Wang et al. Caspase-1 inhibitor ameliorates experimental autoimmune myasthenia gravis by innate dendric cell IL-1-IL-17 pathway. (2015) J.Neuroinflammatio. 12:118} {ELISA} {}
affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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