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文献和实验Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates
selenide/zinc sulfide core-shell nanocrystals, or quantum dots, have greater photostability, resist metabolic and chemical degradation, are nontoxic, and display broad emission and narrow excitation bands. When conjugated to generic adaptor molecules
conjugated to epidermal growth factor act as functional ligands for their receptor, erbB1. Here, we describe protocols for (1) conjugation of streptavidin-QDs to biotinylated ligand, (2) formation of ligand-QD-receptor complexes, and (3) quantification
?streptavidin conjugates are synthesized by covalent coupling of thiol?modified DNA oligonucleotides and streptavidin (STV). The resulting conjugates have binding capacities for four equivalents of biotin and a complementary nucleic acid sequence. The conjugates
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