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100ul
| 产品编号 | bsm-33012M-HRP |
| 英文名称 | T7 tag Mouse mAb, HRP conjugated |
| 中文名称 | HRP标记的T7 tag(标签)单克隆抗体 |
| 英文别名 | T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag. |
| 产品应用 | WB=1:1000-5000 Not yet tested in other applications. |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide T7-tag |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
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文献和实验. For alkaline phosphatase, use a 1:3000 dilution of Biorad goat anti-mouse or anti-rabbit alkaline phosphatase conjugated antibody in blotto. Incubate on rocker 1 hr at 25℃. 8. Wash 3X3min in TBS/Tween. 9. Alkaline phosphatase development: Add developing
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
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