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- bs-33272R
- 2025年10月01日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-33272R |
| 英文名称 | Alpha-actinin-1 Rabbit pAb |
| 中文名称 | α-辅肌动蛋白1抗体 |
| 英文别名 | ACTN 1; ACTN1_HUMAN; alpha Actinin 1; alpha actinin cytoskeletal isoform; alpha-actinin cytoskeletal isoform; alpha-actinin-1; FLJ40884; FLJ54432; Actinin alpha 1; F-Actin Cross-Linking Protein; Non-Muscle alpha-Actinin-1; Actinin, alpha 1; Actinin 1 Smooth Muscle; BDPLT15; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | Recombinant human Alpha-actinin-1 protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 103 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Lineage specification > Mesoderm Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Crosslinking Stem Cells > Lineage Markers > Mesoderm |
| 亚基 | Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN (By similarity). |
| 亚细胞定位 | Cytoplasm > cytoskeleton. _x000D_ Cytoplasm > myofibril > sarcomere > Z line. _x000D_ Cell membrane. _x000D_ Cell junction. _x000D_ Cell projection › ruffle. |
| 相似性 | Belongs to the alpha-actinin family. _x000D_ Contains 1 actin-binding domain. _x000D_ Contains 2 CH (calponin-homology) domains. _x000D_ Contains 2 EF-hand domains. _x000D_ Contains 4 spectrin repeats. |
| 功能 | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
25 ug total protein per lane of various lysates (see on figure) probed with Alpha-actinin-1 polyclonal antibody, unconjugated (bs-33272R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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Alpha-actinin-1 Rabbit pAb(bs-33272R)-50ul/100ul/200ul
￥1180 - 2800
