纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
表位:
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
免疫原:
Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.
描述:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
细胞定位:
Endoplasmic reticulum membrane. Microsome membrane.
保存说明:
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol.
Application: WB; Species:None Available; Sample:None Available;
WBApplication: None Available; Species:None Available; Sample:None Available;
Application: IF/ICC; Species:None Available; Sample:None Available;
IF/ICCApplication: None Available; Species:None Available; Sample:None Available;