- ￥800 - 2500
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- bsm-62229R
- 2025年10月01日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/25ul
| 规格： | 50ul | 产品价格： | ￥1400.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2500.0 |
| 规格： | 25ul | 产品价格： | ￥800.0 |
| 产品编号 | bsm-62229R |
| 英文名称 | CD150/SLAM Recombinant Rabbit mAb |
| 中文名称 | 信号淋巴细胞活化分子CD150重组兔单抗 |
| 英文别名 | CD150; CDw150; SLAM; SLAMF1. |
| 产品应用 | WB=1:500-2000, IP=1:20-50, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | A synthesized peptide derived from human SLAMF1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Recombinant |
| 理论分子量 | 37 |
| 实际分子量 | 85 |
| 储存液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究领域 | Cardiovascular > Angiogenesis > Endothelial Cell Markers Immunology > Cell Type Markers > CD > Non-lineage Microbiology > Interspecies Interaction > Host Virus Interaction Stem Cells > Hematopoietic Progenitors > Hematopoietic Stem Cells > HSC markers Stem Cells > Hematopoietic Progenitors > Surface Molecules |
| 亚基 | nteracts (via cytoplasmic domain) with SH2D1A, and withPTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus Hprotein and acts as a receptor for this virus. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Present on the surface of B-cells and T-cells. |
| 组织特异性 | Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. |
| 翻译后修饰 | Phosphorylated by FYN. |
| 相似性 | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. _x000D_ Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IP} | {1:20-50} |
| {Flow-Cyt} | {1ug/Test} |
25 ug total protein per lane of various lysates (see on figure) probed with CD150/SLAM monoclonal antibody, unconjugated (bsm-62229R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD150/SLAM antibody (bsm-62229R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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CD150/SLAM Recombinant Rabbit mAb(bsm-62229R)-50ul/100ul/25ul
￥800 - 2500
