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NF-L Recombinant Rabbit mAb(bs

m-41217R)-25ul/50ul/100ul
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  • ￥800 - 2500
  • Bioss已认证
  • bsm-41217R
  • 2025年10月01日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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    • 规格：

      25ul/50ul/100ul

    规格：25ul产品价格：￥800.0
    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    产品编号bsm-41217R
    英文名称NF-L Recombinant Rabbit mAb
    中文名称低分子量神经丝蛋白重组兔单抗
    英文别名Neurofilament L; Neurofilament 68; Neurofilament triplet L; 70 kD Neurofilament Light; 68kDa neurofilament protein; CMT 1F; CMT 2E; CMT1F; CMT2E; FLJ53642; Light molecular weight neurofilament protein; NEFL; Neurofilament light; Neurofilament light polypeptide 68kDa; Neurofilament light polypeptide; Neurofilament protein, light chain; Neurofilament subunit NF L; Neurofilament triplet L protein; NF 68; NF L; NF68; NFL; NFL_HUMAN.
    产品应用WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原Recombinant human NF-L protein
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量68 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Developmental Biology > Lineage specification > Ectoderm

    Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class IV > Neurofilaments

    Stem Cells > Lineage Markers > Ectoderm

    亚基Interacts with ARHGEF28. Interacts with TRIM2.
    翻译后修饰O-glycosylated.
    Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
    Ubiquitinated in the presence of TRIM2 and UBE2D1.
    相似性Belongs to the intermediate filament family.
    功能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Neurofilament light polypeptide also called NF-L; Neurofilament triplet L protein; 68 kDa neurofilament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Belongs to the intermediate filament family.

     

    应用推荐稀释比例
    {WB}{1:500-5000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    (Negative control) P‌‌araformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片2
    (Negative control) P‌‌araformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    (Negative control) P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-L Monoclonal Antibody, Unconjugated(bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片11
    25 ug total protein per lane of various lysates (see on figure) probed with NF-L monoclonal antibody, unconjugated (bsm-41217R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片12
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NF-L Monoclonal Antibody, Unconjugated (bsm-41217R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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