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- bs-6382R-HRP
- 2025年09月30日
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100ul
| 产品编号 | bs-6382R-HRP |
| 英文名称 | PTK5/FRK Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的胃肠道相关酪氨酸激酶/蛋白酪氨酸激酶5抗体 |
| 英文别名 | beta cell Src homology tyrosine kinase; BSK; BSK/IYK; C85044; Frk; Frk fyn related kinase; FRK_HUMAN; FYN related kinase; FYN-related kinase; GASK; Gastrointestinal associated kinase; Gastrointestinal associated tyrosine kinase; GTK; Intestine tyrosine kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5; PTK5; RAK; Tyrosine protein kinase FRK; Tyrosine-protein kinase FRK. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FRK/PTK5 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Cycle Inhibitors Cell Biology > Cell Cycle > Kinases/Phosphatases |
| 亚基 | Interacts (via the SH3-domain) with PTEN. Interacts with RB1. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Predominantly found in the nucleus, with a small fraction found in the cell periphery. |
| 组织特异性 | Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon. |
| 相似性 | Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. |
| 功能 | Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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