Phospho1 Rabbit pAb, Biotin conjugated(bs-12562R-Bio)-100ul

Phospho1 Rabbit pAb, Biotin co

njugated(bs-12562R-Bio)-100ul
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  • ¥2980
  • Bioss已认证
  • bs-12562R-Bio
  • 2025年09月30日
  • 产品信息以Bioss网站为准
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      100ul

    产品编号bs-12562R-Bio
    英文名称Phospho1 Rabbit pAb, Biotin conjugated
    中文名称Biotin标记的细胞质磷酸酶1抗体
    英文别名EC 3.1.3.75; PHOP1_HUMAN; Phosphatase orphan 1; Phosphatase, orphan 1; phospho 1; phosphoethanolamine/phosphocholine phosphatase.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Phospho1
    亚型IgG
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > Structures > Bone

    Stem Cells > Mesenchymal Stem Cells > Chondrogenesis

    Stem Cells > Mesenchymal Stem Cells > Osteogenesis

    组织特异性Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
    相似性Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
    功能Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
    保存条件Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

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