Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Uterus tissue lysates
Lane 4: Rat Rectum tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Spleen tissue lysates
Lane 8: Human HL60 cell lysates
Lane 9: Human Huvec cell lysates
Lane 10: Human 293T cell lysates
Lane 11: Human Jurkat cell lysates
Lane 12: Human A549 cell lysates
Primary: Anti-beta-Actin/HRP (bs-0061R-HRP) at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
- ￥1200
- Bioss
- bs-0061R-HRP
- 2025年10月15日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-0061R-HRP |
| 英文名称 | beta-Actin Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的β-肌动蛋白/β-Actin（内参）抗体 |
| 英文别名 | beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; beta actin; beta cytoskeletal actin; A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN. |
| 中文别名 | β actin; βactin; |
| 产品应用 | WB=1:5000-50000, IHC-P=1:100-500, IHC-F=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Isotype/Loading Controls > Loading Controls > Beta Actin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Tags & Cell Markers > Subcellular Markers > Cytoskeleton > Actin |
| 亚基 | Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 组织特异性 | Ubiquitously expressed in all eukaryotic cells. |
| 翻译后修饰 | ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. |
| 相似性 | Belongs to the actin family. |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:5000-50000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 3.361 }}] {Tang Q et al. Maresin1 regulates neutrophil recruitment and IL-10 expression in Aspergillus fumigatus keratitis. Int Immunopharmacol. 2019 Apr;69:103-108.} {WB} {Mouse}
相关实验
affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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beta-Actin Rabbit pAb, HRP conjugated(bs-0061R-HRP)-100ul
￥1200
