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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human Thrombomodulin / CD141
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Mouse Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF, FC
- 靶点:
Uniprot:P15306
- 抗体英文名:
Thrombomodulin / CD141 Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:105,70kDa
应用详情:WB: 1:50-1:200
IF:1:50-1:200
FC: 1:50-1:200
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文献和实验CD3/ CD4 /CD8 T cell subset counts
are not going to be analyzed immediately, steps 1-4 are performed and the samples are stored at 40C in dark until further analysis. Step 5 is performed just before analysis. Recipes 1. Round bottom tube (capacity 5 ml) 2. Test antibody (CD3-FITC/ CD
-cryopreserved) Apheresis Cells 1) Dilute PBMC to 5-10 x 106 cells/ml in Incomplete Medium (pre-warmed to 37°C). 2) Wash Dynabeads® ClinExVivo™ Epoxy as described above for Dynabeads® CD3⁄CD28 CTS™. 3) Add Dynabeads®
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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