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- 详细信息
- 文献和实验
- 技术资料
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu Ms Rat
- 保质期:
12 months
- 抗原来源:
mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
mouse
- 应用范围:
ICC IF
- 靶点:
Uniprot:P68366
- 抗体英文名:
alpha Tubulin mouse Monoclonal Antibody FITC Conjugated
- 规格:
50ul/100ul
计算分子量:50
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:ICC=1:50-200 IF=1:50-200
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
after the Antibody has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d
Conjugation of monoclonal antibodies
in the conjugation of proteins--principally monoclonal antibodies--to fluorescent dyes. These conjugation procedures are commonly performed in our laboratory--we have conjugated several hundred different monoclonals using almost all of the various dyes listed
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