- 询价
- Signalway Antibody
- C92817HRP
- 2025年09月22日
- WB IHC ICC
- mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
mouse
- 应用范围：
WB IHC ICC
- 靶点：
Uniprot：P05362
- 抗体英文名：
ICAM1/CD54 mouse Monoclonal Antibody HRP Conjugated
- 规格：
50ul/100ul
计算分子量:56
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
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文献和实验Monoclonal Antibody Production Protocol
buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). Add 100 ul of substrate to every well. Match color development
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
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