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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthesized peptide derived from human OXGR1
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB;IF
- 靶点:
Uniprot:Q96P68
- 抗体英文名:
OXGR1 Rabbit mAb
- 规格:
50ul/100ul
计算分子量:38 kDa
配方:Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
应用详情:WB 1:1000-1:2000 IF 1:50-1:200
图片:

All lanes : OXGR1 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : A549 whole cell lysates
Lane 3 : HUVEC whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 10 seconds
,

All lanes : OXGR1 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Kidney lysates
Lane 2 : Rat Spleen lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 45 seconds
,

All lanes : OXGR1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : OXGR1 knockdown Hela cell lysate
Lysates/proteins at 20 μg per lane.
,

Immunocytochemistry/ Immunofluorescence OXGR1 antibody (60143)
60143 staining OXGR1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% ParafoX-rmaldehyde permeabilized with 0.1% Triton 100.
Samples were incubated with 60143 at a working dilution of 1/100. The secondary antibody was Alexa Fluor? 488 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control, purified 60143 was used at a dilution of 1/100 followed by Alexa Fluor? 647 goat anti- rabbit antibody at a dilution of 1/500.
Nuclei were counterstained with DAPI.
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文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上,同时减少蛋白不必要降解,这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息:1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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