Recombinant PIVKA-II

Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag

For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions

Recombinant DNA Engineering, Or Cloning Genes In Plasmids

-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection.Transformation The reaction used to create recombinant DNA molecules (ligation reaction, etc.) is used to transform bacteria

II型内含子(group II introns)

主要存在于线粒体中的一类内含子，它的剪接位点类似于核编码结构基因的内含子,并同样遵从GT--AG规律。剪接机理同核内含子的剪接相似,也要形成一个套索的中间体,通过形成5＇-2＇磷酸二酯键将要剪接的位点靠近到一起。但是,II型内含子的剪接又不完全与核内含子的剪接相同,它具有自我剪接的功能,不需要剪接体和snRNA的参与,也不需要ATP供能。从结构上看,II型内含子的6个结构域可形成发夹环, 结构域5与6之间只间隔3个碱基,结构域6参与转酯作用。