- ￥2800
- Proteintech
- CL647-60186
- 2025年10月09日
- FC (Intra)
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cyclin D1 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 647 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
FC (Intra)
- 靶点：
Cyclin D1
- 抗体英文名：
CoraLite® Plus 647-conjugated Cyclin D1 Monoclonal antibody
- 抗体名：
CoraLite® Plus 647-conjugated Cyclin D1 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-60186 targets Cyclin D1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0689 Product name: Recombinant human Cyclin D1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 65-245 aa of BC000076 Sequence: LEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLR |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin D1 |
| 别名 | B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31 |
| 计算分子量 | 295 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000076 |
| 基因名称 | Cyclin D1 |
| Gene ID (NCBI) | 595 |
| RRID | AB_2934982 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P24385 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
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文献和实验Staining Procedure for Flow Cytometric Detection of Human Cyclins
of the anti-cyclin antibodies by flow cytometry. It is a good starting point. However, investigators may need to optimize protocols for their own experimental system. It is particularly useful to refer to the published literature regarding protocols typically
'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
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