- ￥2800
- Proteintech
- CL488-66265
- 2025年10月09日
- WB, IF-P
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human GLS protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 宿主：
Mouse
- 应用范围：
WB, IF-P
- 靶点：
KGA-Specific
- 抗体英文名：
CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66265 targets KGA-Specific in WB, IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag24227 Product name: Recombinant human GLS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 370-669 aa of BC038507 Sequence: YVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutaminase |
| 别名 | AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase |
| 计算分子量 | 669 aa, 73 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC038507 |
| 基因名称 | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_3084228 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O94925 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
conjugated species‐specific secondary antibodies (Invitrogen or Li‐COR, http://www.licor.com) NeutrAvidin, DyLight 800 conjugate (Thermo Scientific
on Veeco DI SPMLab software. 5. Photographs The photographs, showing difference in intensity of Alexa488 signal on glass, SIF, and SACS, were taken with Canon 300D® digital SLR camera. The illumination source was Ti: Sapphire laser (Mai Tai
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
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