相关产品推荐更多 >

FcZero-rAb™ Biotin Anti-Human CD90 Rabbit Recombinant Antibody Biotin-FcA98126
¥750
CoraLite® Plus 488-conjugated GSTP1 单克隆抗体 CL488-66715
¥2800
FcZero-rAb™ CoraLite® Plus 647 Anti-Human CD4 Rabbit Recombinant Antibody CL647-FcA98042
¥480
ZNF828 多克隆抗体 26315-1-AP
¥1350
CoraLite® Plus 488-conjugated BAG6 多克隆抗体 CL488-26417
¥3200
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human KIF23 protein
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human
- 宿主:
Rabbit
- 应用范围:
IF/ICC, Cytometric bead array, Indirect ELISA
- 靶点:
KIF23
- 抗体英文名:
KIF23 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名:
KIF23 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格:
100ug
产品信息
85675-5-PBS targets KIF23 as part of a matched antibody pair:
MP02031-1: 85675-5-PBS capture and 85675-4-PBS detection (validated in Cytometric bead array)
MP02031-2: 85675-5-PBS capture and 85675-3-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | IF/ICC, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag29346 Product name: Recombinant human KIF23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 486-683 aa of NM_138555 Sequence: LDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSV |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | kinesin family member 23 |
| 别名 | 243084E3, KNSL5, MKLP1 |
| 计算分子量 | 110 kDa |
| GenBank蛋白编号 | NM_138555 |
| 基因名称 | KIF23 |
| Gene ID (NCBI) | 9493 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q02241 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7、培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。四、重组病毒鉴定 1、转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2、取30-50% 步骤1中上清,感染50-70%饱和度的25cm2方瓶中293细胞。2-3d后出现明显细胞病变。 3、感染后3-5d,当1/3-1/2细胞漂浮时
-DNA液,加入6ml DMEM完全培养基,37℃孵育过夜,再换液。 7 培养过程中观察细胞生长情况。约2周后可观察到细胞病变(CPE)出现(如用pAdTrack-CMV质粒,由于含GFP,可观察到绿色荧光)。 四 重组病毒鉴定 1 转导10-14d后,收集细胞沉淀,加入2ml灭菌的PBS混悬,冻融细胞,离心后收集上清保存于-80℃。 2 取30-50% 步骤1中上清,感染50-70%饱和度的25cm2 方瓶中293细胞。2-3d后出现明显细胞
的杂交瘤克隆进行筛选,检测它们的特异性和敏感性。ELISA阳性的克隆可用WB检测,筛选出来的克隆至少还需要亚克隆两次。7. 大规模抗体生产:筛选得到两个克隆最后进行大规模培养(每个克隆1L)或者取腹水(每个克隆5只小鼠)。用蛋白A/G对培养液或腹水进行纯化,平均每个克隆可以得到3-5mg 抗体。8. 注意:每个融合平均可以得到900多个克隆,对于多肽抗原可以得到100个左右的ELISA阳性克隆,重组蛋白抗原可以得到100-150个ELISA阳性克隆。亚克隆实验做的越早,越容易保存克隆。因此,为了保存
技术资料暂无技术资料 索取技术资料





