- ￥2800
- Proteintech
- CL488-67974
- 2025年10月09日
- IF/ICC, FC (Intra)
- Mouse
- human, mouse, rat, pig, rabbit
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- 详细信息
- 技术资料
- 免疫原：
Recombinant human LIMK1 protein
- 亚型：
IgG2a
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human, mouse, rat, pig, rabbit
- 宿主：
Mouse
- 应用范围：
IF/ICC, FC (Intra)
- 靶点：
LIMK1
- 抗体英文名：
CoraLite® Plus 488-conjugated LIMK1 Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated LIMK1 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67974 targets LIMK1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag28862 Product name: Recombinant human LIMK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 67-222 aa of BC152982 Sequence: DGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDR |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | LIM domain kinase 1 |
| 别名 | LIMK-1, LIMK 1, LIMK, LIM domain kinase 1, EC:2.7.11.1 |
| 计算分子量 | 647 aa, 73 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC152982 |
| 基因名称 | LIMK1 |
| Gene ID (NCBI) | 3984 |
| RRID | AB_3672967 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P53667 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.
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