- ￥900 - 1800
- Proteintech
- PE-98233
- 2025年10月09日
- FC
- Rabbit
- mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
PE Fluorescent Dye
- 适应物种：
mouse
- 宿主：
Rabbit
- 应用范围：
FC
- 靶点：
CXCR4/CD184
- 抗体英文名：
PE Anti-Mouse CXCR4/CD184 Rabbit Recombinant Antibody
- 抗体名：
PE Anti-Mouse CXCR4/CD184 Rabbit Recombinant Antibody
- 规格：
25ug/100ug
| 规格： | 25ug | 产品价格： | ￥900.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1800.0 |
经过测试的应用
| Positive FC detected in | mouse thymocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-98233 targets CXCR4/CD184 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC |
| 经测试反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) receptor 4 |
| 别名 | 241956H2 |
| 计算分子量 | 40kDa |
| GenBank蛋白编号 | NM_009911.3 |
| 基因名称 | Cxcr4 |
| Gene ID (NCBI) | 12767 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P70658 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
干货 | CUT and Tag 让 ChIP-Seq更简单高效！
说明书推荐的反应体系进行 CUT&Tag 实验；其中，pG-Tn5 转座子使用的终浓度为 0.04μM ，一抗为 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
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